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recombinant murine fgf-basic  (PeproTech)


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    PeproTech recombinant murine fgf-basic
    Recombinant Murine Fgf Basic, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+murine+basic+fgf/basic+fibroblast+growth+factor/pmc12168368-5-0-4
    Average 90 stars, based on 1 article reviews
    recombinant murine fgf-basic - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Mesoderm specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy
    Article Snippet: .. Cells were cultured in 5% CO 2 and maintained in DMEM/F-12 (Life Technologies) supplemented with 15% heat inactivated FBS (Life Technologies), Primocin (100 μg/ml, InvivoGen) and recombinant murine basic FGF (10 ng/ml, Peprotech). .. EMSCs were transduced with lentiviral vector (Origene, PS100085) as described above and stable clones expressing Mest were established using growth medium with 4 μg/ml puromycin.

    Article Title: Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notexin venom Latoxan Cat# L8104 Collagenase I Worthington Biochemical Cat# CLS-1 Collagenase II Worthington Biochemical Cat# CLS-2 Collagenase D Roche Cat# 11 088 858 001 Dispase II Sigma-Aldrich Cat# D4693 (R)-PFI-2 TOCRIS Cat# 4892 (S)-PFI-2 (enantiomer) TOCRIS Cat# 5400 CHIR99021 TOCRIS Cat# 4953 Evans Blue Dye (FW 960.82 g mol 1) Sigma-Aldrich Cat# E2129 Recombinant murine basic FGF Peprotech Cat# 450-33 Recombinant murine Wnt-3a Peprotech Cat# 315-20 Barium Chloride Sigma Cat# B0750 Polybrene Santa Cruz Biotechnology Cat# sc-134220 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNeasy Plus Micro Kit QIAGEN Cat#: 74034 TruSeq stranded mRNA library kit Illumina Cat# RS-122-2101 PureLink Genomic Mini Kit ThermoFisher Cat# K182001 NextSeq 500 High-Output Kit Illumina Cat# FC-404-1002 Click-iT EdU imaging kit ThermoFisher Cat# C10337 Lipofactamine 3000 reagent ThermoFisher Cat# L3000015 DuoLink in situ PLA Kit Sigma Cat# DUO92101 Deposited Data RNaseq: mKO cultured myoblasts This paper SRA: SRP118943 ChIPSeq: H3K4me1 PFI-2 treated myoblasts This paper SRA: SRP118552 ChIPSeq: H3K4me1 mKO cultured myoblasts This paper SRA: SRP118947 Experimental Models: Cell Lines Mouse: C2C12 myoblasts Laboratory of Prof. Helen Blau N/A Experimental Models: Organisms/Strains Mouse: Setd7FL/FL Lehnertz et al., 2011 N/A Mouse: Pax7tm1(Cre)Mrc The Jackson Laboratory JAX: 01530 Mouse: MyoDiCre Chen et al., 2005 N/A Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J The Jackson Laboratory JAX: 012476 Mouse: Pax7-GFP Rocheteau et al., 2012 N/A Mouse: C57BL/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801 Mouse: NOD-scid IL2gmmanull The Jackson Laboratory JAX: 005557 Mouse: B6Ros.Cg-Dmdmdx-4Cv/J The Jackson Laboratory JAX: 002378 Mouse: C57BL/6-Tg(CAG-EGFP)1Osb/J The Jackson Laboratory JAX: 003291 Mouse: FVB-Tg(CAG-Luc,-GFP)L2G85Chco/J The Jackson Laboratory JAX: 008450 Oligonucleotides Setd7 WT primers (exon 7) assay Mm00100479_cn ThermoFisher Cat# 4400291 Setd7 reference primers (exon 1-2) assay Mm00100458_cn modified with VIC dye ThermoFisher Cat# 4442487 Recombinant DNA M50 super 8xTOPFLASH Addgene Cat# 12456 pMSCV-Setd7-3xmyc-IRES-GFP This paper N/A CD51X-GFP-Luc This paper N/A (Continued on next page) e2 Cell Stem Cell 22, 177–190.e1–e7, February 1, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms TopHat2 (Kim et al., 2013) http://ccb.jhu.edu/software/tophat/ index.shtml EdgeR (Robinson et al., 2010) https://bioconductor.org/packages/ release/bioc/html/edgeR.html Genomic Suite ParTek Inc. N/A Burrows-Wheeler Aligner (Li and Durbin, 2009) http://bio-bwa.sourceforge.net/ MACS2 Feng et al., 2012 https://github.com/taoliu/MACS ChAsE (Younesy et al., 2016, 2013) http://chase.cs.univie.ac.at/overview Seqmonk Bioinformatics Group, Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/seqmonk/ Correlation Engine (GO Analysis) NextBio, Ilummina http://www.nextbio.com/b/nextbio.nb VisRSeq (Younesy et al., 2015) http://visrseq.github.io/ Graphpad Prism 6 GraphPad Software https://www.graphpad.com/ Fiji Fiji https://fiji.sc/ FlowJo V10 FlowJo LLC https://www.flowjo.com/solutions/flowjo

    Article Title: Mesoderm-specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy.
    Article Snippet: .. Cells were cultured in 5% CO2 and maintained in DMEM/F-12 (Life Technologies) supplemented with 15% heat inactivated FBS (Life Technologies), Primocin (100 μg/ml, InvivoGen) and recombinant murine basic FGF (10 ng/ml, Peprotech). .. EMSCs were transduced with lentiviral vector (Origene, PS100085) as described above and stable clones expressing Mest were established using growth medium with 4 μg/ml puromycin.

    Recombinant:

    Article Title: Mesoderm specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy
    Article Snippet: .. Cells were cultured in 5% CO 2 and maintained in DMEM/F-12 (Life Technologies) supplemented with 15% heat inactivated FBS (Life Technologies), Primocin (100 μg/ml, InvivoGen) and recombinant murine basic FGF (10 ng/ml, Peprotech). .. EMSCs were transduced with lentiviral vector (Origene, PS100085) as described above and stable clones expressing Mest were established using growth medium with 4 μg/ml puromycin.

    Article Title: Inhibition of methyltransferase Setd7 allows the in vitro expansion of myogenic stem cells with improved therapeutic potential
    Article Snippet: Evans Blue Dye (FW 960.82 g mol −1 ) , Sigma-Aldrich , Cat# E2129. .. Recombinant murine basic FGF , Peprotech , Cat# 450-33. .. Recombinant murine Wnt-3a , Peprotech , Cat# 315-20.

    Article Title: Circulating transforming growth factor-β1 facilitates remyelination in the adult central nervous system
    Article Snippet: Human recombinant TGF-β1 , R and D Systems , Cat. #240-B , . .. Recombinant Murine basic FGF , Peprotech , Cat. #450–33 , . .. Recombinant Murine PDGF-AA , Peprotech , Cat. #315–17 , .

    Article Title: Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notexin venom Latoxan Cat# L8104 Collagenase I Worthington Biochemical Cat# CLS-1 Collagenase II Worthington Biochemical Cat# CLS-2 Collagenase D Roche Cat# 11 088 858 001 Dispase II Sigma-Aldrich Cat# D4693 (R)-PFI-2 TOCRIS Cat# 4892 (S)-PFI-2 (enantiomer) TOCRIS Cat# 5400 CHIR99021 TOCRIS Cat# 4953 Evans Blue Dye (FW 960.82 g mol 1) Sigma-Aldrich Cat# E2129 Recombinant murine basic FGF Peprotech Cat# 450-33 Recombinant murine Wnt-3a Peprotech Cat# 315-20 Barium Chloride Sigma Cat# B0750 Polybrene Santa Cruz Biotechnology Cat# sc-134220 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNeasy Plus Micro Kit QIAGEN Cat#: 74034 TruSeq stranded mRNA library kit Illumina Cat# RS-122-2101 PureLink Genomic Mini Kit ThermoFisher Cat# K182001 NextSeq 500 High-Output Kit Illumina Cat# FC-404-1002 Click-iT EdU imaging kit ThermoFisher Cat# C10337 Lipofactamine 3000 reagent ThermoFisher Cat# L3000015 DuoLink in situ PLA Kit Sigma Cat# DUO92101 Deposited Data RNaseq: mKO cultured myoblasts This paper SRA: SRP118943 ChIPSeq: H3K4me1 PFI-2 treated myoblasts This paper SRA: SRP118552 ChIPSeq: H3K4me1 mKO cultured myoblasts This paper SRA: SRP118947 Experimental Models: Cell Lines Mouse: C2C12 myoblasts Laboratory of Prof. Helen Blau N/A Experimental Models: Organisms/Strains Mouse: Setd7FL/FL Lehnertz et al., 2011 N/A Mouse: Pax7tm1(Cre)Mrc The Jackson Laboratory JAX: 01530 Mouse: MyoDiCre Chen et al., 2005 N/A Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J The Jackson Laboratory JAX: 012476 Mouse: Pax7-GFP Rocheteau et al., 2012 N/A Mouse: C57BL/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801 Mouse: NOD-scid IL2gmmanull The Jackson Laboratory JAX: 005557 Mouse: B6Ros.Cg-Dmdmdx-4Cv/J The Jackson Laboratory JAX: 002378 Mouse: C57BL/6-Tg(CAG-EGFP)1Osb/J The Jackson Laboratory JAX: 003291 Mouse: FVB-Tg(CAG-Luc,-GFP)L2G85Chco/J The Jackson Laboratory JAX: 008450 Oligonucleotides Setd7 WT primers (exon 7) assay Mm00100479_cn ThermoFisher Cat# 4400291 Setd7 reference primers (exon 1-2) assay Mm00100458_cn modified with VIC dye ThermoFisher Cat# 4442487 Recombinant DNA M50 super 8xTOPFLASH Addgene Cat# 12456 pMSCV-Setd7-3xmyc-IRES-GFP This paper N/A CD51X-GFP-Luc This paper N/A (Continued on next page) e2 Cell Stem Cell 22, 177–190.e1–e7, February 1, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms TopHat2 (Kim et al., 2013) http://ccb.jhu.edu/software/tophat/ index.shtml EdgeR (Robinson et al., 2010) https://bioconductor.org/packages/ release/bioc/html/edgeR.html Genomic Suite ParTek Inc. N/A Burrows-Wheeler Aligner (Li and Durbin, 2009) http://bio-bwa.sourceforge.net/ MACS2 Feng et al., 2012 https://github.com/taoliu/MACS ChAsE (Younesy et al., 2016, 2013) http://chase.cs.univie.ac.at/overview Seqmonk Bioinformatics Group, Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/seqmonk/ Correlation Engine (GO Analysis) NextBio, Ilummina http://www.nextbio.com/b/nextbio.nb VisRSeq (Younesy et al., 2015) http://visrseq.github.io/ Graphpad Prism 6 GraphPad Software https://www.graphpad.com/ Fiji Fiji https://fiji.sc/ FlowJo V10 FlowJo LLC https://www.flowjo.com/solutions/flowjo

    Article Title: Mesoderm-specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy.
    Article Snippet: .. Cells were cultured in 5% CO2 and maintained in DMEM/F-12 (Life Technologies) supplemented with 15% heat inactivated FBS (Life Technologies), Primocin (100 μg/ml, InvivoGen) and recombinant murine basic FGF (10 ng/ml, Peprotech). .. EMSCs were transduced with lentiviral vector (Origene, PS100085) as described above and stable clones expressing Mest were established using growth medium with 4 μg/ml puromycin.

    Reporter Assay:

    Article Title: Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notexin venom Latoxan Cat# L8104 Collagenase I Worthington Biochemical Cat# CLS-1 Collagenase II Worthington Biochemical Cat# CLS-2 Collagenase D Roche Cat# 11 088 858 001 Dispase II Sigma-Aldrich Cat# D4693 (R)-PFI-2 TOCRIS Cat# 4892 (S)-PFI-2 (enantiomer) TOCRIS Cat# 5400 CHIR99021 TOCRIS Cat# 4953 Evans Blue Dye (FW 960.82 g mol 1) Sigma-Aldrich Cat# E2129 Recombinant murine basic FGF Peprotech Cat# 450-33 Recombinant murine Wnt-3a Peprotech Cat# 315-20 Barium Chloride Sigma Cat# B0750 Polybrene Santa Cruz Biotechnology Cat# sc-134220 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNeasy Plus Micro Kit QIAGEN Cat#: 74034 TruSeq stranded mRNA library kit Illumina Cat# RS-122-2101 PureLink Genomic Mini Kit ThermoFisher Cat# K182001 NextSeq 500 High-Output Kit Illumina Cat# FC-404-1002 Click-iT EdU imaging kit ThermoFisher Cat# C10337 Lipofactamine 3000 reagent ThermoFisher Cat# L3000015 DuoLink in situ PLA Kit Sigma Cat# DUO92101 Deposited Data RNaseq: mKO cultured myoblasts This paper SRA: SRP118943 ChIPSeq: H3K4me1 PFI-2 treated myoblasts This paper SRA: SRP118552 ChIPSeq: H3K4me1 mKO cultured myoblasts This paper SRA: SRP118947 Experimental Models: Cell Lines Mouse: C2C12 myoblasts Laboratory of Prof. Helen Blau N/A Experimental Models: Organisms/Strains Mouse: Setd7FL/FL Lehnertz et al., 2011 N/A Mouse: Pax7tm1(Cre)Mrc The Jackson Laboratory JAX: 01530 Mouse: MyoDiCre Chen et al., 2005 N/A Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J The Jackson Laboratory JAX: 012476 Mouse: Pax7-GFP Rocheteau et al., 2012 N/A Mouse: C57BL/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801 Mouse: NOD-scid IL2gmmanull The Jackson Laboratory JAX: 005557 Mouse: B6Ros.Cg-Dmdmdx-4Cv/J The Jackson Laboratory JAX: 002378 Mouse: C57BL/6-Tg(CAG-EGFP)1Osb/J The Jackson Laboratory JAX: 003291 Mouse: FVB-Tg(CAG-Luc,-GFP)L2G85Chco/J The Jackson Laboratory JAX: 008450 Oligonucleotides Setd7 WT primers (exon 7) assay Mm00100479_cn ThermoFisher Cat# 4400291 Setd7 reference primers (exon 1-2) assay Mm00100458_cn modified with VIC dye ThermoFisher Cat# 4442487 Recombinant DNA M50 super 8xTOPFLASH Addgene Cat# 12456 pMSCV-Setd7-3xmyc-IRES-GFP This paper N/A CD51X-GFP-Luc This paper N/A (Continued on next page) e2 Cell Stem Cell 22, 177–190.e1–e7, February 1, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms TopHat2 (Kim et al., 2013) http://ccb.jhu.edu/software/tophat/ index.shtml EdgeR (Robinson et al., 2010) https://bioconductor.org/packages/ release/bioc/html/edgeR.html Genomic Suite ParTek Inc. N/A Burrows-Wheeler Aligner (Li and Durbin, 2009) http://bio-bwa.sourceforge.net/ MACS2 Feng et al., 2012 https://github.com/taoliu/MACS ChAsE (Younesy et al., 2016, 2013) http://chase.cs.univie.ac.at/overview Seqmonk Bioinformatics Group, Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/seqmonk/ Correlation Engine (GO Analysis) NextBio, Ilummina http://www.nextbio.com/b/nextbio.nb VisRSeq (Younesy et al., 2015) http://visrseq.github.io/ Graphpad Prism 6 GraphPad Software https://www.graphpad.com/ Fiji Fiji https://fiji.sc/ FlowJo V10 FlowJo LLC https://www.flowjo.com/solutions/flowjo

    Imaging:

    Article Title: Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notexin venom Latoxan Cat# L8104 Collagenase I Worthington Biochemical Cat# CLS-1 Collagenase II Worthington Biochemical Cat# CLS-2 Collagenase D Roche Cat# 11 088 858 001 Dispase II Sigma-Aldrich Cat# D4693 (R)-PFI-2 TOCRIS Cat# 4892 (S)-PFI-2 (enantiomer) TOCRIS Cat# 5400 CHIR99021 TOCRIS Cat# 4953 Evans Blue Dye (FW 960.82 g mol 1) Sigma-Aldrich Cat# E2129 Recombinant murine basic FGF Peprotech Cat# 450-33 Recombinant murine Wnt-3a Peprotech Cat# 315-20 Barium Chloride Sigma Cat# B0750 Polybrene Santa Cruz Biotechnology Cat# sc-134220 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNeasy Plus Micro Kit QIAGEN Cat#: 74034 TruSeq stranded mRNA library kit Illumina Cat# RS-122-2101 PureLink Genomic Mini Kit ThermoFisher Cat# K182001 NextSeq 500 High-Output Kit Illumina Cat# FC-404-1002 Click-iT EdU imaging kit ThermoFisher Cat# C10337 Lipofactamine 3000 reagent ThermoFisher Cat# L3000015 DuoLink in situ PLA Kit Sigma Cat# DUO92101 Deposited Data RNaseq: mKO cultured myoblasts This paper SRA: SRP118943 ChIPSeq: H3K4me1 PFI-2 treated myoblasts This paper SRA: SRP118552 ChIPSeq: H3K4me1 mKO cultured myoblasts This paper SRA: SRP118947 Experimental Models: Cell Lines Mouse: C2C12 myoblasts Laboratory of Prof. Helen Blau N/A Experimental Models: Organisms/Strains Mouse: Setd7FL/FL Lehnertz et al., 2011 N/A Mouse: Pax7tm1(Cre)Mrc The Jackson Laboratory JAX: 01530 Mouse: MyoDiCre Chen et al., 2005 N/A Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J The Jackson Laboratory JAX: 012476 Mouse: Pax7-GFP Rocheteau et al., 2012 N/A Mouse: C57BL/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801 Mouse: NOD-scid IL2gmmanull The Jackson Laboratory JAX: 005557 Mouse: B6Ros.Cg-Dmdmdx-4Cv/J The Jackson Laboratory JAX: 002378 Mouse: C57BL/6-Tg(CAG-EGFP)1Osb/J The Jackson Laboratory JAX: 003291 Mouse: FVB-Tg(CAG-Luc,-GFP)L2G85Chco/J The Jackson Laboratory JAX: 008450 Oligonucleotides Setd7 WT primers (exon 7) assay Mm00100479_cn ThermoFisher Cat# 4400291 Setd7 reference primers (exon 1-2) assay Mm00100458_cn modified with VIC dye ThermoFisher Cat# 4442487 Recombinant DNA M50 super 8xTOPFLASH Addgene Cat# 12456 pMSCV-Setd7-3xmyc-IRES-GFP This paper N/A CD51X-GFP-Luc This paper N/A (Continued on next page) e2 Cell Stem Cell 22, 177–190.e1–e7, February 1, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms TopHat2 (Kim et al., 2013) http://ccb.jhu.edu/software/tophat/ index.shtml EdgeR (Robinson et al., 2010) https://bioconductor.org/packages/ release/bioc/html/edgeR.html Genomic Suite ParTek Inc. N/A Burrows-Wheeler Aligner (Li and Durbin, 2009) http://bio-bwa.sourceforge.net/ MACS2 Feng et al., 2012 https://github.com/taoliu/MACS ChAsE (Younesy et al., 2016, 2013) http://chase.cs.univie.ac.at/overview Seqmonk Bioinformatics Group, Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/seqmonk/ Correlation Engine (GO Analysis) NextBio, Ilummina http://www.nextbio.com/b/nextbio.nb VisRSeq (Younesy et al., 2015) http://visrseq.github.io/ Graphpad Prism 6 GraphPad Software https://www.graphpad.com/ Fiji Fiji https://fiji.sc/ FlowJo V10 FlowJo LLC https://www.flowjo.com/solutions/flowjo

    In Situ:

    Article Title: Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notexin venom Latoxan Cat# L8104 Collagenase I Worthington Biochemical Cat# CLS-1 Collagenase II Worthington Biochemical Cat# CLS-2 Collagenase D Roche Cat# 11 088 858 001 Dispase II Sigma-Aldrich Cat# D4693 (R)-PFI-2 TOCRIS Cat# 4892 (S)-PFI-2 (enantiomer) TOCRIS Cat# 5400 CHIR99021 TOCRIS Cat# 4953 Evans Blue Dye (FW 960.82 g mol 1) Sigma-Aldrich Cat# E2129 Recombinant murine basic FGF Peprotech Cat# 450-33 Recombinant murine Wnt-3a Peprotech Cat# 315-20 Barium Chloride Sigma Cat# B0750 Polybrene Santa Cruz Biotechnology Cat# sc-134220 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNeasy Plus Micro Kit QIAGEN Cat#: 74034 TruSeq stranded mRNA library kit Illumina Cat# RS-122-2101 PureLink Genomic Mini Kit ThermoFisher Cat# K182001 NextSeq 500 High-Output Kit Illumina Cat# FC-404-1002 Click-iT EdU imaging kit ThermoFisher Cat# C10337 Lipofactamine 3000 reagent ThermoFisher Cat# L3000015 DuoLink in situ PLA Kit Sigma Cat# DUO92101 Deposited Data RNaseq: mKO cultured myoblasts This paper SRA: SRP118943 ChIPSeq: H3K4me1 PFI-2 treated myoblasts This paper SRA: SRP118552 ChIPSeq: H3K4me1 mKO cultured myoblasts This paper SRA: SRP118947 Experimental Models: Cell Lines Mouse: C2C12 myoblasts Laboratory of Prof. Helen Blau N/A Experimental Models: Organisms/Strains Mouse: Setd7FL/FL Lehnertz et al., 2011 N/A Mouse: Pax7tm1(Cre)Mrc The Jackson Laboratory JAX: 01530 Mouse: MyoDiCre Chen et al., 2005 N/A Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J The Jackson Laboratory JAX: 012476 Mouse: Pax7-GFP Rocheteau et al., 2012 N/A Mouse: C57BL/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801 Mouse: NOD-scid IL2gmmanull The Jackson Laboratory JAX: 005557 Mouse: B6Ros.Cg-Dmdmdx-4Cv/J The Jackson Laboratory JAX: 002378 Mouse: C57BL/6-Tg(CAG-EGFP)1Osb/J The Jackson Laboratory JAX: 003291 Mouse: FVB-Tg(CAG-Luc,-GFP)L2G85Chco/J The Jackson Laboratory JAX: 008450 Oligonucleotides Setd7 WT primers (exon 7) assay Mm00100479_cn ThermoFisher Cat# 4400291 Setd7 reference primers (exon 1-2) assay Mm00100458_cn modified with VIC dye ThermoFisher Cat# 4442487 Recombinant DNA M50 super 8xTOPFLASH Addgene Cat# 12456 pMSCV-Setd7-3xmyc-IRES-GFP This paper N/A CD51X-GFP-Luc This paper N/A (Continued on next page) e2 Cell Stem Cell 22, 177–190.e1–e7, February 1, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms TopHat2 (Kim et al., 2013) http://ccb.jhu.edu/software/tophat/ index.shtml EdgeR (Robinson et al., 2010) https://bioconductor.org/packages/ release/bioc/html/edgeR.html Genomic Suite ParTek Inc. N/A Burrows-Wheeler Aligner (Li and Durbin, 2009) http://bio-bwa.sourceforge.net/ MACS2 Feng et al., 2012 https://github.com/taoliu/MACS ChAsE (Younesy et al., 2016, 2013) http://chase.cs.univie.ac.at/overview Seqmonk Bioinformatics Group, Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/seqmonk/ Correlation Engine (GO Analysis) NextBio, Ilummina http://www.nextbio.com/b/nextbio.nb VisRSeq (Younesy et al., 2015) http://visrseq.github.io/ Graphpad Prism 6 GraphPad Software https://www.graphpad.com/ Fiji Fiji https://fiji.sc/ FlowJo V10 FlowJo LLC https://www.flowjo.com/solutions/flowjo

    Proximity Ligation Assay:

    Article Title: Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notexin venom Latoxan Cat# L8104 Collagenase I Worthington Biochemical Cat# CLS-1 Collagenase II Worthington Biochemical Cat# CLS-2 Collagenase D Roche Cat# 11 088 858 001 Dispase II Sigma-Aldrich Cat# D4693 (R)-PFI-2 TOCRIS Cat# 4892 (S)-PFI-2 (enantiomer) TOCRIS Cat# 5400 CHIR99021 TOCRIS Cat# 4953 Evans Blue Dye (FW 960.82 g mol 1) Sigma-Aldrich Cat# E2129 Recombinant murine basic FGF Peprotech Cat# 450-33 Recombinant murine Wnt-3a Peprotech Cat# 315-20 Barium Chloride Sigma Cat# B0750 Polybrene Santa Cruz Biotechnology Cat# sc-134220 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNeasy Plus Micro Kit QIAGEN Cat#: 74034 TruSeq stranded mRNA library kit Illumina Cat# RS-122-2101 PureLink Genomic Mini Kit ThermoFisher Cat# K182001 NextSeq 500 High-Output Kit Illumina Cat# FC-404-1002 Click-iT EdU imaging kit ThermoFisher Cat# C10337 Lipofactamine 3000 reagent ThermoFisher Cat# L3000015 DuoLink in situ PLA Kit Sigma Cat# DUO92101 Deposited Data RNaseq: mKO cultured myoblasts This paper SRA: SRP118943 ChIPSeq: H3K4me1 PFI-2 treated myoblasts This paper SRA: SRP118552 ChIPSeq: H3K4me1 mKO cultured myoblasts This paper SRA: SRP118947 Experimental Models: Cell Lines Mouse: C2C12 myoblasts Laboratory of Prof. Helen Blau N/A Experimental Models: Organisms/Strains Mouse: Setd7FL/FL Lehnertz et al., 2011 N/A Mouse: Pax7tm1(Cre)Mrc The Jackson Laboratory JAX: 01530 Mouse: MyoDiCre Chen et al., 2005 N/A Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J The Jackson Laboratory JAX: 012476 Mouse: Pax7-GFP Rocheteau et al., 2012 N/A Mouse: C57BL/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801 Mouse: NOD-scid IL2gmmanull The Jackson Laboratory JAX: 005557 Mouse: B6Ros.Cg-Dmdmdx-4Cv/J The Jackson Laboratory JAX: 002378 Mouse: C57BL/6-Tg(CAG-EGFP)1Osb/J The Jackson Laboratory JAX: 003291 Mouse: FVB-Tg(CAG-Luc,-GFP)L2G85Chco/J The Jackson Laboratory JAX: 008450 Oligonucleotides Setd7 WT primers (exon 7) assay Mm00100479_cn ThermoFisher Cat# 4400291 Setd7 reference primers (exon 1-2) assay Mm00100458_cn modified with VIC dye ThermoFisher Cat# 4442487 Recombinant DNA M50 super 8xTOPFLASH Addgene Cat# 12456 pMSCV-Setd7-3xmyc-IRES-GFP This paper N/A CD51X-GFP-Luc This paper N/A (Continued on next page) e2 Cell Stem Cell 22, 177–190.e1–e7, February 1, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms TopHat2 (Kim et al., 2013) http://ccb.jhu.edu/software/tophat/ index.shtml EdgeR (Robinson et al., 2010) https://bioconductor.org/packages/ release/bioc/html/edgeR.html Genomic Suite ParTek Inc. N/A Burrows-Wheeler Aligner (Li and Durbin, 2009) http://bio-bwa.sourceforge.net/ MACS2 Feng et al., 2012 https://github.com/taoliu/MACS ChAsE (Younesy et al., 2016, 2013) http://chase.cs.univie.ac.at/overview Seqmonk Bioinformatics Group, Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/seqmonk/ Correlation Engine (GO Analysis) NextBio, Ilummina http://www.nextbio.com/b/nextbio.nb VisRSeq (Younesy et al., 2015) http://visrseq.github.io/ Graphpad Prism 6 GraphPad Software https://www.graphpad.com/ Fiji Fiji https://fiji.sc/ FlowJo V10 FlowJo LLC https://www.flowjo.com/solutions/flowjo

    RNA sequencing:

    Article Title: Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notexin venom Latoxan Cat# L8104 Collagenase I Worthington Biochemical Cat# CLS-1 Collagenase II Worthington Biochemical Cat# CLS-2 Collagenase D Roche Cat# 11 088 858 001 Dispase II Sigma-Aldrich Cat# D4693 (R)-PFI-2 TOCRIS Cat# 4892 (S)-PFI-2 (enantiomer) TOCRIS Cat# 5400 CHIR99021 TOCRIS Cat# 4953 Evans Blue Dye (FW 960.82 g mol 1) Sigma-Aldrich Cat# E2129 Recombinant murine basic FGF Peprotech Cat# 450-33 Recombinant murine Wnt-3a Peprotech Cat# 315-20 Barium Chloride Sigma Cat# B0750 Polybrene Santa Cruz Biotechnology Cat# sc-134220 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNeasy Plus Micro Kit QIAGEN Cat#: 74034 TruSeq stranded mRNA library kit Illumina Cat# RS-122-2101 PureLink Genomic Mini Kit ThermoFisher Cat# K182001 NextSeq 500 High-Output Kit Illumina Cat# FC-404-1002 Click-iT EdU imaging kit ThermoFisher Cat# C10337 Lipofactamine 3000 reagent ThermoFisher Cat# L3000015 DuoLink in situ PLA Kit Sigma Cat# DUO92101 Deposited Data RNaseq: mKO cultured myoblasts This paper SRA: SRP118943 ChIPSeq: H3K4me1 PFI-2 treated myoblasts This paper SRA: SRP118552 ChIPSeq: H3K4me1 mKO cultured myoblasts This paper SRA: SRP118947 Experimental Models: Cell Lines Mouse: C2C12 myoblasts Laboratory of Prof. Helen Blau N/A Experimental Models: Organisms/Strains Mouse: Setd7FL/FL Lehnertz et al., 2011 N/A Mouse: Pax7tm1(Cre)Mrc The Jackson Laboratory JAX: 01530 Mouse: MyoDiCre Chen et al., 2005 N/A Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J The Jackson Laboratory JAX: 012476 Mouse: Pax7-GFP Rocheteau et al., 2012 N/A Mouse: C57BL/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801 Mouse: NOD-scid IL2gmmanull The Jackson Laboratory JAX: 005557 Mouse: B6Ros.Cg-Dmdmdx-4Cv/J The Jackson Laboratory JAX: 002378 Mouse: C57BL/6-Tg(CAG-EGFP)1Osb/J The Jackson Laboratory JAX: 003291 Mouse: FVB-Tg(CAG-Luc,-GFP)L2G85Chco/J The Jackson Laboratory JAX: 008450 Oligonucleotides Setd7 WT primers (exon 7) assay Mm00100479_cn ThermoFisher Cat# 4400291 Setd7 reference primers (exon 1-2) assay Mm00100458_cn modified with VIC dye ThermoFisher Cat# 4442487 Recombinant DNA M50 super 8xTOPFLASH Addgene Cat# 12456 pMSCV-Setd7-3xmyc-IRES-GFP This paper N/A CD51X-GFP-Luc This paper N/A (Continued on next page) e2 Cell Stem Cell 22, 177–190.e1–e7, February 1, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms TopHat2 (Kim et al., 2013) http://ccb.jhu.edu/software/tophat/ index.shtml EdgeR (Robinson et al., 2010) https://bioconductor.org/packages/ release/bioc/html/edgeR.html Genomic Suite ParTek Inc. N/A Burrows-Wheeler Aligner (Li and Durbin, 2009) http://bio-bwa.sourceforge.net/ MACS2 Feng et al., 2012 https://github.com/taoliu/MACS ChAsE (Younesy et al., 2016, 2013) http://chase.cs.univie.ac.at/overview Seqmonk Bioinformatics Group, Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/seqmonk/ Correlation Engine (GO Analysis) NextBio, Ilummina http://www.nextbio.com/b/nextbio.nb VisRSeq (Younesy et al., 2015) http://visrseq.github.io/ Graphpad Prism 6 GraphPad Software https://www.graphpad.com/ Fiji Fiji https://fiji.sc/ FlowJo V10 FlowJo LLC https://www.flowjo.com/solutions/flowjo

    Modification:

    Article Title: Inhibition of Methyltransferase Setd7 Allows the In Vitro Expansion of Myogenic Stem Cells with Improved Therapeutic Potential.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notexin venom Latoxan Cat# L8104 Collagenase I Worthington Biochemical Cat# CLS-1 Collagenase II Worthington Biochemical Cat# CLS-2 Collagenase D Roche Cat# 11 088 858 001 Dispase II Sigma-Aldrich Cat# D4693 (R)-PFI-2 TOCRIS Cat# 4892 (S)-PFI-2 (enantiomer) TOCRIS Cat# 5400 CHIR99021 TOCRIS Cat# 4953 Evans Blue Dye (FW 960.82 g mol 1) Sigma-Aldrich Cat# E2129 Recombinant murine basic FGF Peprotech Cat# 450-33 Recombinant murine Wnt-3a Peprotech Cat# 315-20 Barium Chloride Sigma Cat# B0750 Polybrene Santa Cruz Biotechnology Cat# sc-134220 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNeasy Plus Micro Kit QIAGEN Cat#: 74034 TruSeq stranded mRNA library kit Illumina Cat# RS-122-2101 PureLink Genomic Mini Kit ThermoFisher Cat# K182001 NextSeq 500 High-Output Kit Illumina Cat# FC-404-1002 Click-iT EdU imaging kit ThermoFisher Cat# C10337 Lipofactamine 3000 reagent ThermoFisher Cat# L3000015 DuoLink in situ PLA Kit Sigma Cat# DUO92101 Deposited Data RNaseq: mKO cultured myoblasts This paper SRA: SRP118943 ChIPSeq: H3K4me1 PFI-2 treated myoblasts This paper SRA: SRP118552 ChIPSeq: H3K4me1 mKO cultured myoblasts This paper SRA: SRP118947 Experimental Models: Cell Lines Mouse: C2C12 myoblasts Laboratory of Prof. Helen Blau N/A Experimental Models: Organisms/Strains Mouse: Setd7FL/FL Lehnertz et al., 2011 N/A Mouse: Pax7tm1(Cre)Mrc The Jackson Laboratory JAX: 01530 Mouse: MyoDiCre Chen et al., 2005 N/A Mouse: B6;129-Pax7tm2.1(cre/ERT2)Fan/J The Jackson Laboratory JAX: 012476 Mouse: Pax7-GFP Rocheteau et al., 2012 N/A Mouse: C57BL/10ScSn-Dmdmdx/J The Jackson Laboratory JAX: 001801 Mouse: NOD-scid IL2gmmanull The Jackson Laboratory JAX: 005557 Mouse: B6Ros.Cg-Dmdmdx-4Cv/J The Jackson Laboratory JAX: 002378 Mouse: C57BL/6-Tg(CAG-EGFP)1Osb/J The Jackson Laboratory JAX: 003291 Mouse: FVB-Tg(CAG-Luc,-GFP)L2G85Chco/J The Jackson Laboratory JAX: 008450 Oligonucleotides Setd7 WT primers (exon 7) assay Mm00100479_cn ThermoFisher Cat# 4400291 Setd7 reference primers (exon 1-2) assay Mm00100458_cn modified with VIC dye ThermoFisher Cat# 4442487 Recombinant DNA M50 super 8xTOPFLASH Addgene Cat# 12456 pMSCV-Setd7-3xmyc-IRES-GFP This paper N/A CD51X-GFP-Luc This paper N/A (Continued on next page) e2 Cell Stem Cell 22, 177–190.e1–e7, February 1, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms TopHat2 (Kim et al., 2013) http://ccb.jhu.edu/software/tophat/ index.shtml EdgeR (Robinson et al., 2010) https://bioconductor.org/packages/ release/bioc/html/edgeR.html Genomic Suite ParTek Inc. N/A Burrows-Wheeler Aligner (Li and Durbin, 2009) http://bio-bwa.sourceforge.net/ MACS2 Feng et al., 2012 https://github.com/taoliu/MACS ChAsE (Younesy et al., 2016, 2013) http://chase.cs.univie.ac.at/overview Seqmonk Bioinformatics Group, Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/seqmonk/ Correlation Engine (GO Analysis) NextBio, Ilummina http://www.nextbio.com/b/nextbio.nb VisRSeq (Younesy et al., 2015) http://visrseq.github.io/ Graphpad Prism 6 GraphPad Software https://www.graphpad.com/ Fiji Fiji https://fiji.sc/ FlowJo V10 FlowJo LLC https://www.flowjo.com/solutions/flowjo



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    Journal: Cell reports

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    doi: 10.1016/j.celrep.2024.115101

    Figure Lengend Snippet:

    Article Snippet: The mouse mesenchymal stem cells (EMSC) were cultured in DMEM/F12 (#11330–032, Invitrogen) containing 15% FBS (Corning, #35–015-CV), 100 μL Primocin (#ant-pm-1, Invivogen) per 50 mL media, and 10 ng/mL recombinant murine FGF-basic (#450–33, Peprotech).

    Techniques: Virus, Recombinant, Magnetic Beads, Control, Negative Control, Ubiquitin Proteomics, shRNA, Plasmid Preparation, Immunofluorescence, Software

    Journal: iScience

    Article Title: Tumor-derived colorectal cancer organoids induce a unique Treg cell population by directing CD4 + T cell differentiation

    doi: 10.1016/j.isci.2025.111827

    Figure Lengend Snippet:

    Article Snippet: Recombinant murine FGF-basic , PeproTech , 450-33.

    Techniques: Recombinant, SYBR Green Assay, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Staining, RNA Sequencing, Transgenic Assay, Software, Cell Culture, Membrane, Sterility, Pore Size

    FGFR4 inhibition abrogates cardiac myocyte hypertrophy induced by serum from fed pythons. Neonatal rat ventricular myocytes (NRVMs) were treated with media containing either vehicle, FGF2 (25 ng/mL), FGF23 (25 ng/mL), or serum from fasted or fed snakes (diluted to 2% of the total media volume), without or in combination with a small molecule inhibitor of FGFR4 (iFGFR4; BLU9931, 10 ng/mL), for 48 h. Compared to vehicle-treated NRVMs (Ctrl) or to NRVMs treated with serum from fasted pythons, serum collected from pythons twelve hours post-feeding (Python 12HPF; #### p < 0.0001) and three days post-feeding (Python 3DPF; #### p < 0.0001) induced a significant increase in myocyte area. When co-treated with iFGFR4 (textured bars), both Python 12HPF (**** p < 0.0001) and Python 3DPF (*** p = 0.0003) did not induce a significant increase in NRVM area when compared to the same treatment without iFGFR4. Python 12HPF ( $$$ p = 0.0003)- and Python 3DPF ( $$ p = 0.0016)-treated NRVMs had significantly increased myocyte area when compared to Python Fasted serum treatments. Treatment with recombinant FGF23 significantly increased myocyte area compared to Ctrl ( #### p < 0.0001), fasted python serum treatments ( $$$$ p < 0.0001), and FGF23 + iFGFR4 treatment (**** p < 0.0001) NRVMs. Treatment with recombinant FGF2 protein served as a positive control for hypertrophy ( ## p = 0.003). Treatment with serum from fasted and two-days-post-feeding water snakes (Water Snake Fasted, Water Snake 2DPF) served as negative controls Each individual color corresponds with the treatment indicated below the bars. # = versus Ctrl; * = versus same treatment + iFGFR4; $ = versus Python Fasted; 150 cells per condition; n = 3–4 independent isolations of NRVMs. All values are shown as mean ± SD.

    Journal: Journal of Cardiovascular Development and Disease

    Article Title: FGFR4 Is Required for Concentric Growth of Cardiac Myocytes during Physiologic Cardiac Hypertrophy

    doi: 10.3390/jcdd11100320

    Figure Lengend Snippet: FGFR4 inhibition abrogates cardiac myocyte hypertrophy induced by serum from fed pythons. Neonatal rat ventricular myocytes (NRVMs) were treated with media containing either vehicle, FGF2 (25 ng/mL), FGF23 (25 ng/mL), or serum from fasted or fed snakes (diluted to 2% of the total media volume), without or in combination with a small molecule inhibitor of FGFR4 (iFGFR4; BLU9931, 10 ng/mL), for 48 h. Compared to vehicle-treated NRVMs (Ctrl) or to NRVMs treated with serum from fasted pythons, serum collected from pythons twelve hours post-feeding (Python 12HPF; #### p < 0.0001) and three days post-feeding (Python 3DPF; #### p < 0.0001) induced a significant increase in myocyte area. When co-treated with iFGFR4 (textured bars), both Python 12HPF (**** p < 0.0001) and Python 3DPF (*** p = 0.0003) did not induce a significant increase in NRVM area when compared to the same treatment without iFGFR4. Python 12HPF ( $$$ p = 0.0003)- and Python 3DPF ( $$ p = 0.0016)-treated NRVMs had significantly increased myocyte area when compared to Python Fasted serum treatments. Treatment with recombinant FGF23 significantly increased myocyte area compared to Ctrl ( #### p < 0.0001), fasted python serum treatments ( $$$$ p < 0.0001), and FGF23 + iFGFR4 treatment (**** p < 0.0001) NRVMs. Treatment with recombinant FGF2 protein served as a positive control for hypertrophy ( ## p = 0.003). Treatment with serum from fasted and two-days-post-feeding water snakes (Water Snake Fasted, Water Snake 2DPF) served as negative controls Each individual color corresponds with the treatment indicated below the bars. # = versus Ctrl; * = versus same treatment + iFGFR4; $ = versus Python Fasted; 150 cells per condition; n = 3–4 independent isolations of NRVMs. All values are shown as mean ± SD.

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    Techniques: Inhibition, Recombinant, Positive Control